Thermo Fisher Scientific FAK Monoclonal Antibody (ZF002)
FAK 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체(ZF002). 인간, 마우스, 랫트에 반응하며 WB, IHC, ICC, ELISA에 적합. 단백질 A로 정제된 액상형 항체로 안정적이며 다양한 형광 표지 형식 제공.
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Thermo Fisher Scientific FAK Monoclonal Antibody (ZF002)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 8 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:500 | - |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL | View 4 publications |
| ELISA | 0.1–1 µg/mL | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ZF002 |
| Immunogen | Recombinant protein derived from the N-terminal region of the mouse and rat FAK-1 proteins |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533427 |
Available Conjugated Formats
Product Specific Information
Mouse and rat FAK-1 proteins share 93% homology with human. On western blots, this antibody identifies the target band at approximately 110 kDa. Reactivity has been confirmed with HeLa cell lysates and mouse and rat brain homogenates by western blotting.
Target Information
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase acting as a substrate for Src and a key element of integrin signaling. It plays an essential role in cell spreading, differentiation, migration, cell death, and the G1–S phase transition of the cell cycle. FAK localizes to focal adhesions, sites where cells attach to the extracellular matrix via integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of Grb2 and c-Src with FAK, resulting in activation of ERK2 MAP kinase. In v-Src-transformed NIH3T3 cells, the association of v-Src, Grb2, and Sos with FAK is independent of adhesion. The Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK at Tyr925. Several transcript variants encoding different isoforms have been identified for the FAK gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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