
Thermo Fisher Scientific GFP Recombinant Rabbit Monoclonal Antibody
GFP 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체로, 높은 특이성과 일관된 로트 간 재현성을 제공합니다. WB, IHC, ICC, IP 등 다양한 응용에 적합하며, 동물 유래 성분이 없는 포뮬레이션으로 연구 신뢰성을 향상시킵니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:20–1:200 | 29 |
| Immunohistochemistry (IHC) | 1:20–1:200 | 82 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 4 |
| Immunohistochemistry - Free Floating (IHC (Free)) | - | 1 |
| Immunocytochemistry (ICC/IF) | 1:100 | 30 |
| Flow Cytometry (Flow) | Assay-dependent | 3 |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | 25 |
| ChIP assay (ChIP) | - | 2 |
| in situ PLA (PLA) | - | 1 |
| Immunomicroscopy (IM) | - | 2 |
| In Situ Hybridization (ISH) | - | 1 |
| Affinity Purification (AP) | - | 2 |
| In vitro Assay (IV) | - | 2 |
| Miscellaneous PubMed (Misc) | - | 6 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Pig, Tag |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Immunogen | Full-length GFP |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2536526 |
Product Specific Information
Storage and Reconstitution:
To prepare a 0.2 mg/mL stock solution, reconstitute the lyophilized antibody in 0.5 mL PBS (pH 7.4).
Reconstituted product can be stored for up to 3 months at 4°C with the addition of 2 mM sodium azide.Recombinant Antibody Production:
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal clones for production.
Advantages include:- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
The green fluorescent protein (GFP) from Aequorea victoria emits light in bioluminescence reactions and is widely used as a reporter protein in both eukaryotic and prokaryotic systems. As a fusion tag, GFP enables localization and tracking of proteins, and the study of subcellular dynamics.
- Molecular Weight: 27 kDa (monomeric)
- Absorption: 395 nm (blue light)
- Emission: 508 nm (green light)
- Properties: Stable, species-independent fluorescence suitable for diverse applications
- Applications: Gene expression monitoring, protein localization, interaction studies (e.g., FRET, yeast two-hybrid)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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