Thermo Fisher Scientific GFP Recombinant Rabbit Monoclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:20-1:200

View 29 publications 29 publications

Immunohistochemistry (IHC)

1:20-1:200

View 82 publications 82 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 4 publications 4 publications

Immunohistochemistry - Free Floating (IHC (Free))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 30 publications 30 publications

Flow Cytometry (Flow)

Assay-dependent

View 3 publications 3 publications

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

View 25 publications 25 publications

ChIP assay (ChIP)

-

View 2 publications 2 publications

in situ PLA (PLA)

-

View 1 publication 1 publication

Immunomicroscopy (IM)

-

View 2 publications 2 publications

In Situ Hybridization (ISH)

-

View 1 publication 1 publication

Affinity Purification (AP)

-

View 2 publications 2 publications

In vitro Assay (IV)

-

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 6 publications 6 publications

Product Specifications

Species Reactivity

Tag

Published species

Pig, Tag

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Immunogen

Full-length GFP

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

-20° C, store in dark

Shipping conditions

Ambient

RRID

AB_2536526

Product Specific Information

Storage and reconstitution: to prepare a 0.2 mg/mL stock solution, reconstitute the lyophilized antibody in 0.5 mL PBS, pH 7.4. Reconstituted product can be stored for up to 3 months at 4ºC with the addition of 2mM sodium azide.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.