Thermo Fisher Scientific Cyclin B1 Monoclonal Antibody (2E6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

2E6

Immunogen

Recombinant human Cyclin B1 protein, around N-terminal 150aa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cyclin B1,uniProtId:P14635-1,ncbiNodeId:9606,antigenRange:1-150,antigenLength:433,antigenImageFileName:BSM-52044R_Cyclin_B1_P14635-1_Rabbit.svg,antigenImageFileNamePDP:BSM-52044R_Cyclin_B1_P14635-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

Target Information

Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.