Thermo Fisher Scientific HIF1A Recombinant Rabbit Monoclonal Antibody (16H4L13)
HIF1A 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, ICC/IF, ELISA, ChIP 등 다양한 응용에 적합합니다. 고특이성과 일관된 로트 간 재현성을 제공하며, 인간 시료에 반응하고 마우스에도 예측 반응합니다. 단기 4°C, 장기 -20°C 보관 권장.
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- 700505
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:5,000 | View 5 publications |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | View 2 publications |
| ELISA | Assay-dependent | - |
| ChIP assay (ChIP) | 1 µL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 16H4L13 |
| Immunogen | Proprietary |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532327 |
Product Specific Information
- This antibody is predicted to react with mouse based on sequence homology.
- Intact IgG appears on a non-reducing gel as a ~150 kDa band and upon reduction generates ~25 kDa (light chain) and ~50 kDa (heavy chain) bands.
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems.
- Antibody DNA sequences from immunoreactive rabbits are cloned and expressed.
- Individual clones are screened to select optimal candidates for production.
- Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor found in mammalian cells under hypoxic conditions. HIF-1 consists of alpha and beta subunits, both belonging to the basic-helix-loop-helix PAS family of transcription factors.
HIF1 functions as a transcriptional regulator of adaptive responses to hypoxia, activating over 40 genes including erythropoietin, glucose transporters, glycolytic enzymes, VEGF, and HILPDA. These genes enhance oxygen delivery and metabolic adaptation under low oxygen conditions.
HIF1-alpha also regulates hypoxia-mediated apoptosis, cell proliferation, and tumor angiogenesis. Under hypoxia, p53 protein accumulation interacts with HIF1-alpha, promoting MDM2-mediated ubiquitination and degradation of HIF1-alpha. Induction of NOX4 by HIF1-alpha helps maintain ROS levels and supports hypoxia-induced proliferation.
In humans, HIF1A is located on chromosome 14q. The C-terminal binds to p300, forming p300/CBP-HIF complexes that induce hypoxia-responsive genes such as VEGF. Hypoxia plays a critical role in diseases like myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease, and COPD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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