Thermo Fisher Scientific HIF1A Recombinant Rabbit Monoclonal Antibody (16H4L13)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• This antibody is predicted to react with mouse based on sequence homology.
• Intact IgG appears on a non-reducing gel as a ~150 kDa band and upon reduction generates ~25 kDa (light chain) and ~50 kDa (heavy chain) bands.
• Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems.
  • Antibody DNA sequences from immunoreactive rabbits are cloned and expressed.
  • Individual clones are screened to select optimal candidates for production.
• Advantages of recombinant rabbit monoclonal antibodies:
  • High specificity and sensitivity
  • Lot-to-lot consistency
  • Animal origin-free formulation
  • Broad immunoreactivity due to the rabbit immune repertoire

Target Information

HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor found in mammalian cells under hypoxic conditions. HIF-1 consists of alpha and beta subunits, both belonging to the basic-helix-loop-helix PAS family of transcription factors.

HIF1 functions as a transcriptional regulator of adaptive responses to hypoxia, activating over 40 genes including erythropoietin, glucose transporters, glycolytic enzymes, VEGF, and HILPDA. These genes enhance oxygen delivery and metabolic adaptation under low oxygen conditions.

HIF1-alpha also regulates hypoxia-mediated apoptosis, cell proliferation, and tumor angiogenesis. Under hypoxia, p53 protein accumulation interacts with HIF1-alpha, promoting MDM2-mediated ubiquitination and degradation of HIF1-alpha. Induction of NOX4 by HIF1-alpha helps maintain ROS levels and supports hypoxia-induced proliferation.

In humans, HIF1A is located on chromosome 14q. The C-terminal binds to p300, forming p300/CBP-HIF complexes that induce hypoxia-responsive genes such as VEGF. Hypoxia plays a critical role in diseases like myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease, and COPD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.