Thermo Fisher Scientific cKit Polyclonal Antibody, FITC
FITC 형광 표지된 c-Kit 폴리클로날 항체로 다양한 종에서 반응하며, Western blot, ELISA, IP 등에 사용 가능. 친화 크로마토그래피로 정제되어 높은 특이성과 안정성을 제공. 연구용으로 hematopoietic 및 stem cell 관련 단백질 분석에 적합.
- 카탈로그번호
- CKIT-101-FITC
- 판매단위
- pk
카탈로그
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Thermo Fisher Scientific cKit Polyclonal Antibody, FITC
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Non-human primate, Pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 130–180 on human Tyrosine-Kinase c-Kit protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. Together with its ligand, KIT regulates growth and activation of various hemopoietic and non-hemopoietic cells.
Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has been a focal point in therapeutic strategies for neoplastic mast cell growth.
c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract cajal cells. Upon binding of stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of tyrosine residues, including tyrosine 703. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma, and JAK/STAT.
c-kit receptor activation is essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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