Thermo Fisher Scientific Phospho-gamma-H2AX (Ser139) Polyclonal Antibody
DNA 손상 지표인 γ-H2AX(Ser139)을 인식하는 rabbit polyclonal 항체. Western blot, IHC, ICC/IF에 적합하며 높은 민감도와 특이성을 제공. 항원 친화 크로마토그래피로 정제된 액상 형태로, 4°C 보관 및 wet ice 배송.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:2,000–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region surrounding phosphorylated serine 140 of human histone H2AX |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Notes:
- For ICC: Formaldehyde fixation and permeabilization with Triton-X 100 are recommended.
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
H2AX is a variant of the H2A histone family and a component of the nucleosome core. It becomes phosphorylated by kinases such as ATM and ATR in the PI3K pathway. Phosphorylated γ-H2AX marks the initiation of DNA double-strand break (DSB) repair by recruiting DNA repair proteins.
γ-H2AX foci form rapidly in response to DSBs induced by ionizing radiation or cytotoxic agents, serving as a biomarker for DNA damage. Visualization of γ-H2AX enables assessment of DNA damage, repair activity, and genomic stability.
This antibody is useful for detecting DNA damage caused by chemical, environmental, or physical stressors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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