Thermo Fisher Scientific Phospho-gamma-H2AX (Ser139) Polyclonal Antibody
DNA 손상 마커인 γ-H2AX(Ser139)을 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC 등 다양한 응용에 사용 가능. 고감도 검출 및 DNA 복구 연구에 적합. 1 mg/mL 액상 형태로 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:2,000–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region surrounding phosphorylated serine 140 of human histone H2AX |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Notes:
- For ICC, formaldehyde fixation and permeabilization with Triton-X 100 are recommended.
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
H2AX is a variant of the H2A protein family and a component of the histone octamer in nucleosomes. It is phosphorylated by kinases such as ATM and ATR in the PI3K pathway.
Phosphorylated H2AX (γ-H2AX) is a key marker for DNA double-strand breaks (DSBs), facilitating the recruitment of DNA repair proteins.
γ-H2AX foci formation corresponds directly to DSBs, making it a reliable biomarker for DNA damage caused by ionizing radiation, cytotoxic agents, or environmental stress.
This antibody is widely used for assessing genomic damage and studying DNA repair mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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