Thermo Fisher Scientific P4HTM Polyclonal Antibody
Thermo Fisher Scientific의 P4HTM Polyclonal Antibody는 HIF Prolyl Hydroxylase 4를 인식하는 Rabbit IgG 항체입니다. Western blot 및 IHC(Paraffin)에서 검증되었으며, 높은 특이성과 재현성을 제공합니다. 연구용으로 사용되며, 산소 항상성 관련 단백질 분석에 적합합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:100–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to a region within amino acids 317–512 of Human HIF Prolyl Hydroxylase 4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.88 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2548227 |
Product Specific Information
- Recommended positive controls: A549, HeLa
- Predicted reactivity: Mouse (84%), Bovine (83%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis.
Prolyl hydroxylase is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, prolyl hydroxylases convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels reduce this conversion, stabilizing HIF and increasing angiogenesis.
Prolyl hydroxylase 4 specifically catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It may function as a cellular oxygen sensor and, under normoxic conditions, target HIF for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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