Thermo Fisher Scientific P4HTM Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:1,000

Product Specifications

Product Specific Information

• Recommended positive controls: A549, HeLa
• Predicted reactivity: Mouse (84%), Bovine (83%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis.

Prolyl hydroxylase is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, prolyl hydroxylases convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels reduce this conversion, stabilizing HIF and increasing angiogenesis.

Prolyl hydroxylase 4 specifically catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It may function as a cellular oxygen sensor and, under normoxic conditions, target HIF for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.