Thermo Fisher Scientific CD40 Monoclonal Antibody (1C10), PE, eBioscience
마우스 CD40 항원을 인식하는 단클론 항체로, PE 형광 표지되어 Flow Cytometry 분석에 최적화됨. B 세포 활성 및 면역 반응 연구에 적합하며, 0.5 µg/test로 사용 가능. 4°C 보관, 동결 금지.
- 카탈로그번호
- 12-0401-8x (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD40 Monoclonal Antibody (1C10), PE, eBioscience
Thermo Fisher Scientific CD40 Monoclonal Antibody (1C10), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.5 µg/test | View 74 publications |
| Neutralization (Neu) | - | View 1 publication |
| Inhibition Assays (IA) | - | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1C10 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465648 |
Available Formats
- Unconjugated
- APC
- Biotin
- Brilliant UV 661
- Brilliant Violet 605
- Functional Grade
- PE-Cyanine5
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 780
- Request custom conjugation
Product Specific Information
The 1C10 monoclonal antibody reacts with mouse CD40, a 45–50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells.
CD40 regulates B cell development and maturation by inducing Ig isotype switching and, together with IL-4, protects B cells from surface Ig-induced apoptosis while promoting proliferation.
Interaction of CD40 with CD154 (gp39), its ligand on T cells, is critical for T–B cell crosstalk, costimulation, and immune regulation.
The 1C10 antibody is reported to be agonistic in vitro and in vivo.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.5 µg/test (100 µL final volume). Optimal titration recommended.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD40 antigen is a single-chain glycoprotein belonging to the TNF/NGF superfamily, homologous to CD30. It is expressed on B cells (except plasma cells), epithelial cells, carcinomas, and lymphoid dendritic cells.
CD40 mediates immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
AKNA transcription factor regulates CD40 expression, while TNFR2 acts as a signal transduction adaptor.
CD40–CD154 interaction is implicated in amyloid-beta-induced microglial activation, related to Alzheimer’s disease pathogenesis.
Two alternatively spliced transcript variants encoding distinct isoforms are known.
Diseases associated with CD40 dysfunction include Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.