Thermo Fisher Scientific XPF Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Product Specific Information

Antibody detects endogenous levels of total XPF.

Target Information

The structure-specific ERCC1/XPF endonuclease complex is involved in the repair of two distinct types of DNA lesions:

• Nucleotide excision repair (NER) for UV-induced lesions and bulky chemical adducts
• Recombination repair of genotoxic interstrand cross-links

NER involves dual incisions on both sides of the DNA damage catalyzed by two nucleases. In mammalian cells, XPG cleaves the 3′ side of the lesion, while ERCC1/XPF makes the 5′ incision. ERCC1/XPF also likely plays a role in processing partially repaired recombination intermediates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.