Thermo Fisher Scientific MBD2 Polyclonal Antibody
Rabbit polyclonal antibody against MBD2 protein. Validated for WB and ELISA in mouse and rat samples. Affinity purified and supplied as liquid in PBS with 50% glycerol. Recommended storage at -20°C. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 202–411 of human MBD2 (NP_003918.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.12 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914375 |
Product Specific Information
- Positive test controls: mouse heart, mouse kidney, rat heart
- Subcellular localization: Nucleus
Immunogen sequence:
DLSSFDFRTG KMMPSKLQKN KQRLRNDPLN QNKGKPDLNT TLPIRQTASI FKQPVTKVTN HPSNKVKSDP QRMNEQPRQL FWEKRLQGLS ASDVTEQIIK TMELPKGLQG VGPGSNDETL LSAVASALHT SSAPITGQVS AAVEKNPAVW LNTSQPLCKA FIVTDEDIRK QEERVQQVRK KLEEALMADI LSRAADTEEM DIEMDSGDEA
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. Mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of adaptor molecules involved in gene silencing and chromatin structure. High-affinity recognition of methylated histone H3 by HP1 requires a functional chromodomain, which interacts specifically with the methyl epitope on lysine-9. In vivo, heterochromatin association of HP1 is lost in Suv39h double mutant mouse fibroblasts but restored upon reintroduction of catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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