Thermo Fisher Scientific CD41a Monoclonal Antibody (HIP8), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 6 publications

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Product Specifications

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Additional Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 660-120S
• NovaFluor Yellow 590
• NovaFluor Yellow 660
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

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Product Specific Information

Description:
The HIP8 monoclonal antibody reacts with the human CD41 molecule (integrin alpha IIb, platelet GPIIb). CD41 associates with integrin beta3 (CD61) and is expressed by megakaryocytes and platelets. The CD41/CD61 complex acts as a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin, and thrombospondin, mediating platelet aggregation. HIP8 blocks platelet aggregation.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of human platelets. Use at 5 µL (0.125 µg) per test. One test = amount of antibody to stain a cell sample in 100 µL volume. Cell number range: 10⁵–10⁸ cells/test.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples can be stored in:

• IC Fixation Buffer (Product #00-8222-49)
• 1-step Fix/Lyse Solution (Product #00-5333-54)
  for up to 3 days at 4°C in the dark with minimal impact on brightness and compensation. Clone-specific performance should be determined empirically.

Excitation / Emission / Laser:

• Excitation: 488–561 nm
• Emission: 775 nm
• Compatible Lasers: Blue, Green, Yellow-Green

Filtration:
0.2 µm post-manufacturing filtered.

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Target Information

CD41 (platelet glycoprotein IIb, ITGA2B):
Composed of two subunits (α: 120 kDa, β: 23 kDa) that interact with CD61 in the presence of calcium to form a functional receptor. Involved in platelet aggregation and adhesion via binding to von Willebrand factor, fibrinogen, fibronectin, and vitronectin. Expressed mainly on megakaryocyte-platelet lineage and during early hematopoietic differentiation.
Associated diseases include Glanzmann Thrombasthenia and Platelet type-16 bleeding disorder.

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For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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