Thermo Fisher Scientific Vinculin Monoclonal Antibody (VCL, 2572)
Human Vinculin(VCL)에 특이적인 Mouse Monoclonal Antibody로 Western blot, IHC, Peptide Array에 사용 가능. Recombinant fragment(aa174-322) 기반 면역원 사용. Protein A/G 정제, PBS buffer, 무보존제, -20°C 또는 -80°C 보관. 세포 부착 및 F-actin 연결 연구에 적합.
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- 7414-MSM1-P1ABX
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL | - |
| Peptide Array (Array) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VCL, 2572 |
| Immunogen | Recombinant fragment (around aa 174–322) of human Vinculin (VCL) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C followed by cooling at room temperature for 20 minutes.
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane.
In adhesion plaque assembly, the beta subunit of integrin binds to talin, which binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system.
Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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