Thermo Fisher Scientific Vinculin Monoclonal Antibody (VCL, 2572)
Vinculin 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, IHC(P), Peptide Array에 사용 가능. Protein A/G 정제, 200 µg/mL 농도. Human 시료에 반응하며 세포 부착 관련 연구에 적합. 연구용으로만 사용.
- 카탈로그번호
- 7414-MSM1-P1
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL | - |
| Peptide Array (Array) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VCL, 2572 |
| Immunogen | Recombinant fragment (around aa 174–322) of human Vinculin (VCL) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed):
Incubate antibody for 30 min at room temperature (RT).
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at RT for 20 minutes.
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell–cell and cell–extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. In adhesion plaque assembly, the beta subunit of integrin binds to talin, which binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds and cross-links actin filaments, vinculin plays a key role in linking the extracellular matrix to the cytoplasmic microfilament system.
Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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