Thermo Fisher Scientific Vinculin Monoclonal Antibody (VCL, 2572)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed):
  Incubate antibody for 30 min at room temperature (RT).
  Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at RT for 20 minutes.

Target Information

Vinculin (VCL) is a cytoskeletal protein associated with cell–cell and cell–extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. In adhesion plaque assembly, the beta subunit of integrin binds to talin, which binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds and cross-links actin filaments, vinculin plays a key role in linking the extracellular matrix to the cytoplasmic microfilament system.
Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.