Thermo Fisher Scientific CD133 (Prominin-1) Monoclonal Antibody (13A4), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• Alexa Fluor 488
• APC
• Biotin
• FITC
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Custom conjugation available upon request

Product Specific Information

The 13A4 monoclonal antibody recognizes mouse Prominin-1 (CD133, human orthologue AC133), a 115–120 kDa pentaspan transmembrane glycoprotein. Prominin-1 is expressed on primitive cells such as hematopoietic stem and progenitor cells, neural and endothelial stem cells, retina, retinoblastoma, and developing epithelium. The function and ligand of Prominin-1 remain unknown. The 13A4 antibody does not cross-react with rat, human, chicken, or Drosophila antigens but has been reported to work in canine samples.

Applications Reported:

• Flow cytometry: ~0.25–1 µg per million cells
• Immunoprecipitation: ~10–25 µg/mL
• Western blot: ~1–5 µg/mL
• Immunohistochemistry: ~1–10 µg/mL

Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells; effective at ≤1 µg/test (in 100 µL final volume). Optimal titration recommended.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD133 (AC133) is a 97 kDa glycoprotein with five transmembrane domains, appearing as a 120 kDa band on gels due to glycosylation. It is a cell surface marker expressed by immature hematopoietic stem cells but not by mature blood cells. CD133 is also expressed in neural and embryonic stem cells, and in certain cancers such as leukemias and brain tumors. Low-level expression occurs in kidney, pancreas, placenta, and fetal liver. Mutations in CD133 are associated with retinitis pigmentosa and Stargardt disease. Expression is regulated by multiple tissue-dependent promoters and alternative transcripts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.