Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), PE-eFluor 610, eBioscience

Applications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 660
• Alexa Fluor 700
• Alexa Fluor Plus 594
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450 / 506 / 570 / 615 / 660
• FITC
• PE
• PE-Cyanine5 / PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein present during active phases of the cell cycle (G1, S, G2, and mitosis), but absent in resting cells (G0). Ki-67 serves as a proliferation marker, useful for determining the growth fraction of cell populations and predicting tumor outcomes.

SolA15 also recognizes human, non-human primate, and canine Ki-67.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00). Recommended ≤0.125 µg/test (100 µL final volume; 10⁵–10⁸ cells/test). Optimize titration for best performance.

Fluorophore Information:
PE-eFluor® 610 is excited by 488–561 nm lasers and emits at 607 nm. Use 610/20 band pass filter (PE-Texas Red® equivalent). Protect from light due to photo-induced oxidation sensitivity.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) up to 3 days at 4°C in the dark with minimal fluorescence loss. Performance may vary by clone.

Excitation: 488–561 nm
Emission: 607 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin and heterochromatin regions through its FHA domain. Ki-67 is a standard proliferation marker absent in G0 cells and correlates with tumor grade and cell growth fraction. It is widely used in oncology and neuroscience research for assessing cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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