Thermo Fisher Scientific PKC epsilon Polyclonal Antibody
PKC epsilon 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot 및 IHC(Paraffin) 검증 완료. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관. 인간 및 마우스 시료에 반응하며, 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,500–1:5,000 | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human PRKCE. Recombinant protein control fragment (Product #RP-102967) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2790878 |
Product Specific Information
Immunogen sequence:
PPFKPRIKTK RDVNNFDQDF TREEPVLTLV DEAIVKQINQ EEFKGFSYFG EDLMP
Target Information
The PKC family of serine/threonine kinases, including PRKCE (PKC epsilon), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity.
PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are involved in diverse cellular signaling pathways. They also serve as major receptors for phorbol esters, a class of tumor promoters. Each PKC isoform has distinct cellular roles and expression profiles.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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