Thermo Fisher Scientific WTAP Polyclonal Antibody
WTAP 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 응용에 사용 가능. Human 반응성, 항원 친화 정제, 액상 형태이며 4°C 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 | |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 | |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 346 and 396 of Human Wilms tumor 1 associated protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with X. laevis and X. tropicalis
Target Information
Wilms’ tumor (WT) is an embryonal malignancy of the kidney that affects approximately 1 in 10,000 infants. The Wilms’ tumor protein (WT1) binds the DNA sequence GCGGGGGCG and functions as a transcriptional repressor. WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may regulate mRNA splicing. WTAP localizes to nuclear speckles and regulates the G2/M cell cycle transition by binding to the 3′ UTR of cyclin A2, enhancing its stability. It also inhibits WT1 target gene expression and impairs WT1 DNA-binding ability. Two isoforms exist due to alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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