Thermo Fisher Scientific WTAP Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: Assay-dependent

Immunoprecipitation (IP)

• Tested Dilution: 2–5 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt

Target Information

Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects approximately 1 in 10,000 infants and occurs in both sporadic and inherited forms.
The Wilms' tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn²⁺ finger transcriptional activators, and functions as a transcriptional repressor.
WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may be involved in regulating mRNA splicing.
WTAP is localized in nuclear speckles and regulates the G2/M cell cycle transition by binding to the 3′ UTR of cyclin A2, enhancing its stability.
Additionally, WTAP inhibits expression of WT1 target genes and can impair WT1 DNA-binding ability.
Two isoforms of WTAP exist due to alternative splicing events.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.