
Thermo Fisher Scientific ATG14 Recombinant Superclonal Antibody
ATG14 단백질을 인식하는 Thermo Fisher Scientific의 재조합 Superclonal 항체. Western blot 및 ICC/IF에 적합하며, 높은 특이성과 재현성을 제공. 인간에 반응하며, 단기 4°C, 장기 -20°C 보관. 단백질 A 정제 및 PBS 완충액 포함.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–3 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Antibody |
| Immunogen | 3 synthetic peptides corresponding to human ATG14 (aa 63–79, 162–178, 277–293) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532756 |
Product Specific Information
This antibody is predicted to react with Monkey, Rat, Mouse, Pig, Bovine, Rabbit, and Dog.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They combine multiple recombinant monoclonal antibodies, providing both the sensitivity of polyclonal antibodies and the specificity of monoclonal antibodies, with the consistency of recombinant production.
These antibodies recognize multiple epitope sites, ensuring higher detection sensitivity for low abundance targets. Each lot maintains a consistent antibody population, eliminating biological variability.
Note: Formerly called “Recombinant polyclonal antibody”, now rebranded as “Recombinant Superclonal™ antibody”. The product and performance remain unchanged.
Target Information
ATG14 (Autophagy related protein homolog 14) is encoded by the ATG14 gene located on human chromosome 14. Also known as ATG14L or BARKOR (Beclin 1-associated autophagy-related key regulator), ATG14 plays a key role in autophagy — a conserved process for recycling misfolded proteins and degrading dysfunctional organelles.
Autophagy is induced under nutrient stress, leading to autophagosome formation and fusion with lysosomes. The Class III PI3-Kinase complex, including Vps34 and Beclin 1, interacts with ATG14 (type 1) or UVRAG (type 2). ATG14 directs the type 1 complex to the preautophagosomal structure (PAS), which is critical for autophagosome initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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