Thermo Fisher Scientific GUK1 Monoclonal Antibody (1H3)
GUK1 단백질을 인식하는 Mouse IgM 단일클론 항체로, Western blot, ELISA, Immunoprecipitation에 적합합니다. 인간 단백질에 반응하며 비결합형 액상 형태로 제공됩니다. -20°C에서 보관하며 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:500–1:1,000 |
| Immunoprecipitation (IP) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H3 |
| Immunogen | GUK1 (NP_000849, 100–197 a.a.) partial recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Storage Buffer | Ascites |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Protein sequence:
LDVDLQGVRN IKATDLRPIY ISVQPPSLHV LEQRLRQRNT ETEESLVKRL AAAQADMESS KEPGLFDVVI INDSLDQAYA ELKEALSEEI KKAQRTGA
Target Information
Guanylate kinase (GK) catalyzes the ATP-dependent phosphorylation of GMP into GDP, essential for recycling GMP and indirectly cGMP. It is a conserved monomeric protein (~200 amino acids) found in prokaryotes, lower eukaryotes, and vertebrates. GK shares structural similarity with protein A57R (SalG2R) from Vaccinia virus strains.
Proteins containing DHR, SH3, and C-terminal GK-like domains are termed MAGUKs (membrane-associated guanylate kinase homologs) and include:
- Drosophila lethal(1)discs large-1 tumor suppressor protein (dlg1)
- Mammalian tight junction protein Zo-1
- Synaptic proteins (SAP90/PSD-95, CHAPSYN-110/PSD-93, SAP97/DLG1, SAP102)
- Vertebrate 55 kDa erythrocyte membrane protein (p55)
- C. elegans protein lin-2
- Rat protein CASK
- Human proteins DLG2 and DLG3
GK possesses an ATP-binding site (P-loop) in its N-terminal region, not conserved in GK-like domains of MAGUKs. However, these proteins retain residues involved in MP binding.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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