Thermo Fisher Scientific NES Monoclonal Antibody (OTI3B12), TrueMAB
Human Nestin 단백질(aa 99-349)에 특이적인 마우스 단클론 항체. Western blot 및 IHC(P)에서 검증됨. 액상 형태로 1 mg/mL 농도, PBS/BSA/glycerol buffer에 보관. 줄기세포 및 종양 내 신생혈관 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3B12 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 99–349 of human NES produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | –20 °C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. It is widely used as a marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Nestin serves as an angiogenic marker to evaluate neovascularity in tumors. Highly specific antibodies to human Nestin can help characterize progenitor cell differentiation, enhance therapeutic potential in CNS disease models, and identify neuroepithelial tumor cells. Nestin expression is also observed in pancreatic and hematopoietic stem cell populations and is considered a novel angiogenesis marker in proliferating endothelial cells of colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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