Thermo Fisher Scientific VEGF Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

The VEGF-A monoclonal antibody clone #16F1 (Product #M808) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody P802B (biotinylated conjugate of Product #P802).
Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125–0.5 µg/mL for detection.

Target Information

VEGF (vascular endothelial growth factor) is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis, promoting tumor progression and metastasis.
It enhances endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability.
The VEGF family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF, which regulate angiogenesis through receptor systems VEGFR-1 (flt-1) and VEGFR-2 (flk-1/KDR).
Alternative splicing generates five isoforms of VEGF, each binding specifically to these tyrosine kinase receptors expressed on endothelial cells.
An alternately spliced form of FLT1 produces a soluble protein, sFLT1, that binds VEGF with high affinity and inhibits angiogenesis.
Elevated VEGF levels are associated with POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes), also known as Crow-Fukase syndrome, affecting multiple organs.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.