Thermo Fisher Scientific DYKDDDDK Tag Monoclonal Antibody (5A8E5), THE
DYKDDDDK(FALG) 태그 단백질 검출용 고친화도 Mouse Monoclonal Antibody(5A8E5). Western blot, ICC/IF, Flow cytometry, ELISA, IP 등 다양한 응용 가능. Lyophilized 형태로 제공되며, Protein A 정제 및 PBS buffer 포함.
- 카탈로그번호
- A00187xxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific DYKDDDDK Tag Monoclonal Antibody (5A8E5), THE
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1.0 µg/mL |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
| Flow Cytometry (Flow) | 1 µg/mL |
| ELISA | 0.05–0.2 µg/mL |
| Immunoprecipitation (IP) | 1 µg/mL |
| Miscellaneous PubMed (Misc) | – |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Not Applicable |
| Host/Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5A8E5 |
| Immunogen | Synthetic peptide (DYKDDDDK) coupled to KLH |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats Available
- Biotin
- FITC
- HRP
- iFluor 488
- iFluor 555
- iFluor 647
- Custom conjugation available upon request
Product Specific Information
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
The DYKDDDDK peptide is a small epitope tag that does not interfere with bioactivity or biodistribution of recombinant proteins. It is widely used as a general epitope tag in expression vectors.
THE™ DYKDDDDK Tag Antibody, mAb, Mouse is a high-affinity monoclonal antibody suitable for detecting DYKDDDDK-tagged proteins.
Target Information
The DYKDDDDK tag (FLAG tag) is an eight-amino-acid sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) commonly used for protein purification, detection, and localization. It can be fused to either terminus of a protein, enabling easy identification and isolation using anti-FLAG antibodies.
This tag is hydrophilic and less likely to denature proteins compared to other epitope tags.
Widely used in molecular biology and biochemistry for:
- Protein-protein interaction studies
- Protein expression analysis
- Protein localization studies
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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