Thermo Fisher Scientific DYKDDDDK Tag Monoclonal Antibody (5A8E5), THE

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1.0 µg/mL

-

Immunocytochemistry (ICC/IF)

1 µg/mL

-

Flow Cytometry (Flow)

1 µg/mL

-

ELISA (ELISA)

0.05-0.2 µg/mL

-

Immunoprecipitation (IP)

1 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 26 publications 26 publications

Product Specifications

Species Reactivity

Tag

Published species

Not Applicable

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

5A8E5

Immunogen

A synthetic peptide (DYKDDDDK) coupled to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• FITC
• HRP
• iFluor 488
• iFluor 555
• iFluor 647
• Request custom conjugation

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. THE™ DYKDDDDK Tag Antibody, mAb, Mouse is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins.

Target Information

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.