Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, AP
염기성 인산분해효소(AP) 결합 염소 유래 항-토끼 IgG (H+L) 2차 항체로, WB, IHC, ELISA, IP 등 다양한 응용에 적합. 친화 크로마토그래피로 정제되어 높은 특이성과 민감도를 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| ELISA | 1:1,000 |
| Immunoprecipitation (IP) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulin |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.05M Tris, pH 7.5, with 50% glycerol, 1 mM MgCl₂, 1% BSA, 0.15M NaCl |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533968 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Conjugate Formats
- Alexa Fluor 488-10 nm colloidal gold
- Alexa Fluor 488-5 nm colloidal gold
- Biotin
- DyLight 350
- DyLight 405
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 633
- DyLight 650
- DyLight 680
- DyLight 800 4X PEG
- FITC
- HRP
- PE-Cyanine5.5
- TRITC
- WesternDot 625
- Request custom conjugation available
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of their specific targets. Secondary antibodies enhance versatility by allowing use with various detection systems such as HRP, AP, or fluorescence. They also improve sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. Typically, these antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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