Thermo Fisher Scientific CD63 Monoclonal Antibody (LAMP3, 2790)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

CD63 (LAMP-3, lysosome-associated membrane protein-3) is a glycoprotein of the tetraspanin family, present in late endosomes, lysosomes, and secretory vesicles of various cell types. CD63 is also found on the plasma membrane, typically following cell activation, and is widely used as a basophil activation marker.
In mast cells, CD63 exposure does not require activation. It interacts with integrins, affects phagocytosis and cell migration, and is involved in H/K-ATPase trafficking and regulation of ROMK1 channels. CD63 also serves as a T-cell co-stimulation molecule.
Expression of CD63 can be used to predict prognosis in early-stage carcinomas. It is expressed on activated platelets, monocytes, macrophages, and weakly on granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen ME491 and the platelet antigen PTLGP40.
Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.