Thermo Fisher Scientific p38 MAPK Recombinant Rabbit Monoclonal Antibody (23GB4540)

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:5,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:1,000

Flow Cytometry (Flow)

• Tested Dilution: 1:2,000

Product Specifications

Target Information

The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in its nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motif. There are four isoforms of p38 MAPK, differing in tissue expression and affinity for upstream activators and downstream effectors. Upon exposure to tumor necrosis factor, interleukin-1, heat shock, or other stimuli, MAPK kinase-3 is activated by phosphorylation. Activated MAPK kinase-3/6 phosphorylate Thr180 and Tyr182 residues in p38 MAPK. Phospho-p38 MAPK subsequently activates transcription factors such as ATF-2, CHOP-1, and MEF-2 through phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.