Thermo Fisher Scientific PSMB7 Monoclonal Antibody (OTI4D12), TrueMAB
Human 및 Mouse에서 반응하는 PSMB7 단클론 항체로 Western blot에 최적화. 항원은 E.coli에서 생산된 인간 PSMB7 단백질(aa 58–277) 기반. 무보존제, 동결건조 형태로 제공되며, 재구성 및 결합 실험에 적합. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4D12 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 58–277 of human PSMB7 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure composed of four rings of 28 non-identical subunits (two α-rings and two β-rings).
Proteasomes are abundant in eukaryotic cells and cleave peptides in an ATP/ubiquitin-dependent, non-lysosomal pathway.
The immunoproteasome plays a crucial role in processing class I MHC peptides.
PSMB7 encodes a member of the proteasome B-type (T1B) family, a 20S core β subunit of the proteasome. Expression is downregulated by gamma interferon, and proteolytic processing is required for maturation. This subunit is absent in the immunoproteasome and replaced by catalytic subunit 2i (proteasome β10).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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