Thermo Fisher Scientific eEF2 Polyclonal Antibody
Rabbit polyclonal antibody targeting human eEF2 region (residues 808–858). Suitable for WB, IHC, IHC(P), and IP applications. Recommended shelf life 1 year; storage at 4°C. Predicted cross-reactivity with Rat, Chicken, Bovine, and Orangutan.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 808 and 858 of human eukaryotic translation elongation factor 2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, antibody predicted to react with Rat, Chicken, Bovine, and Orangutan
Target Information
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. In this process, the ribosome transitions from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. This enzyme coordinates the movement of tRNA molecules, mRNA, and conformational changes in the ribosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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