Thermo Fisher Scientific eEF2 Kinase Polyclonal Antibody
eEF2 kinase를 표적으로 하는 Thermo Fisher Scientific의 rabbit polyclonal antibody. Western blot, IHC, IP 등 다양한 응용에 적합하며, 인간과 마우스 시료에 반응. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 | |
| Immunohistochemistry (IHC) | 1:500–1:2,000 | |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500–1:2,000 | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 675 and 725 of human calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase (EEF2K). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Rat.
Target Information
EEF2K (Eukaryotic Elongation Factor 2 Kinase) is a highly conserved serine/threonine kinase involved in the calmodulin signaling pathway. Its activity is increased in several cancers, including breast cancer. EEF2K regulates protein synthesis by controlling peptide chain elongation through phosphorylation of elongation factor EEF2, rendering EEF2 inactive and reducing the rate of protein synthesis. Activation occurs via upstream kinases such as AMPK or TRPM7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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