Thermo Fisher Scientific Histone H1 Recombinant Rabbit Monoclonal Antibody (AE-4)
Histone H1 표적의 재조합 토끼 단일클론 항체(AE-4)로, Western blot, IHC, ICC, Flow cytometry에 적합. 인간, 마우스, 랫트 반응성. HEK293 발현 시스템에서 생산되며 Protein A로 정제된 액상 형태. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | AE-4 |
| Immunogen | Nuclei of human leukemia biopsy cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.
Target Information
This locus is the ortholog of a mouse protein-coding gene that displays characteristics of a linker histone and is expressed in nuclei of late maturing spermatids. The human locus is expressed; however, the open reading frame has been disrupted by a frameshift so it is unlikely to encode a functional protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: 3005-RBM5-P0_Histone_H1_P16401-1_Rabbit.svg, 3005-RBM5-P0_Histone_H1_P16401-1_Rabbit_PDP.jpeg, 3005-RBM5-P0_Histone_H1_P16403-1_Rabbit.svg, 3005-RBM5-P0_Histone_H1_P16403-1_Rabbit_PDP.jpeg, 3005-RBM5-P0_Histone_H1_Q02539-1_Rabbit.svg, 3005-RBM5-P0_Histone_H1_Q02539-1_Rabbit_PDP.jpeg)
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