Thermo Fisher Scientific Aurora B Polyclonal Antibody
Aurora B 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, ICC/IF, IP에 사용 가능. 인간 Aurora B kinase의 C-말단 영역(300-344 aa)을 면역원으로 제작. 액상(Unconjugated) 형태, 1 mg/mL 농도, 4°C 보관. 연구용 전용 시약.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 300 and the C-terminus (residue 344) of human Aurora B kinase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Aurora kinases associate with microtubules during chromosome movement and segregation.
AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to K-fibers.
This complex functions in chromosome alignment and separation, histone modification, and cytokinesis.
The Chromosomal Passenger Complex (CPC) includes:
- Aurora B kinase (AurB)
- Inner centromere protein (INCENP)
- Survivin
- Borealin
These subunits determine the activity, localization, stability, and substrate specificity of AurB.
Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and AurB.
AurB is overexpressed in multiple tumor types, including primary breast and colon tumors, and may interact with Aurora-A kinase through a feedback mechanism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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