Thermo Fisher Scientific RIT1 Polyclonal Antibody
RIT1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용 가능. Human, Mouse, Rat 반응성. Protein A 정제, 고농도(1 mg/mL) 액상 형태, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human RIT1, amino acids 141–219 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Rit and its neuron-specific homologue, Rin, define a subfamily of Ras-related GTPases. Both are membrane-associated despite lacking a CAAX box or similar lipidation motif. Rit and Rin share 64% amino acid sequence identity and a conserved nine amino acid effector domain (DPTIEDAYK) between murine and human proteins. They interact with Ras-binding proteins such as RalGDS, Rlf, and AF-6, but not with Raf kinases, RIN1, or PI3 kinase p110 subunit. These findings suggest Rit and Rin regulate signaling pathways distinct from those controlled by Ras.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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