Thermo Fisher Scientific MAP2K1 Monoclonal Antibody (OTI9E9), TrueMAB
MAP2K1(MEK1) 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, IHC, ICC 등 다양한 응용에 사용 가능. 인간 유래 항원 기반으로 제작되었으며, 고순도의 친화 크로마토그래피 정제 제품. 동결건조 형태로 안정적 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:8000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9E9 |
| Immunogen | Full length human recombinant protein of human MAP2K1 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase involved in the MAPK signaling cascade, regulating cell growth and differentiation.
It is activated by various growth factors, cytokines, and calcium influx, and phosphorylated at serine residues 218 and 222 by Raf1.
MAP2K1 plays roles in stress response, apoptosis, and cytokine-induced proliferation.
Mutations in MAP2K1 are associated with cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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