Thermo Fisher Scientific MAP2K1 Monoclonal Antibody (OTI6C6), TrueMAB
MAP2K1(MEK1) 단백질을 표적하는 mouse IgG1 단일클론 항체로, WB, IHC, ICC 등 다양한 응용에 적합. 고순도 친화 크로마토그래피 정제, 무보존제 제형. 세포 성장 및 분화 연구용으로 최적화된 연구 전용 항체.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:4000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6C6 |
| Immunogen | Full length human recombinant protein of human MAP2K1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to reach a final antibody concentration of about 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase involved in mitogen-activated protein kinase (MAPK) cascades, regulating cell growth and differentiation.
Activated by various growth factors, cytokines, and calcium influx, MEK1 is phosphorylated at serine residues 218 and 222 by Raf1.
It plays roles in stress response, apoptosis, and cytokine-induced proliferation.
Mutations in MAP2K1 can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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