Thermo Fisher Scientific HCoV-229E Spike S2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

ELISA

• Tested Concentration: 1 µg/mL

Product Specifications

Product Specific Information

• Positive test controls include: Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECDHis Tag).
• Immunogen sequence:
  LENKSAELNY TVQKLQTLID NINSTLVDLK WLNRVETYIK WPWWVWLCIS VVLIFVVSML LLCCCSTGCC GFFSCFASSI RGCCESTKLP YYDVEKIHIQ

Target Information

The S1 region attaches the virion to the cell membrane by interacting with host ANPEP (aminopeptidase N), initiating infection. Binding to the receptor induces conformational changes in the S glycoprotein, exposing the fusion peptide of the S2 region and activating membrane fusion.
The S2 region belongs to the class I viral fusion proteins and has at least three conformational states: pre-fusion native, pre-hairpin intermediate, and post-fusion hairpin.
During viral and host membrane fusion, the coiled-coil (heptad repeat) regions form a trimer-of-hairpins structure, positioning the fusion peptide near the C-terminal ectodomain region. This structural rearrangement drives membrane apposition and fusion. [Uniprot]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.