Thermo Fisher Scientific APC5 Polyclonal Antibody
APC5 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat에 반응합니다. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. Protein A로 정제된 액상 형태이며, 고농도(1 mg/mL)로 안정적인 신호 검출이 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human APC5, amino acids 51–150 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting cell cycle regulators, such as B-type cyclins, for degradation via the 26S proteasome. The encoded protein is essential for proper APC/C ubiquitination function and for interaction with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been identified, resulting in shorter proteins due to translation initiation at downstream AUG sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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