Thermo Fisher Scientific Nestin Polyclonal Antibody
Rabbit polyclonal antibody recognizing human Nestin, a stem and progenitor cell marker. Validated for IHC, ICC/IF, and Flow Cytometry. Supplied as a liquid at 1 mg/mL, unconjugated. Suitable for research use in stem cell and neurobiology studies.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within Human Nestin amino acids 1471–1520 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2802815 |
Target Information
Nestin is a Class VI intermediate filament protein abundantly expressed in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. It serves as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Nestin is also a superior angiogenic marker for evaluating neovascularity in tumor endothelial cells. Antibodies specific to human Nestin are valuable for characterizing progenitor cells differentiating into distinct lineages, enhancing the therapeutic potential of human neural stem/progenitor cells in CNS disease or injury, and identifying neuroepithelial tumor cells. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker in proliferating endothelial cells of colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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