Thermo Fisher Scientific EPCAM Monoclonal Antibody (OTI1G4), TrueMAB
EpCAM 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI1G4). Western blot과 Immunocytochemistry에 적합하며, Human 시료 반응. Lyophilized 형태로 제공되며, PBS buffer에 trehalose 포함. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G4 |
| Immunogen | Full length human recombinant protein of human EPCAM produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues.
It functions as a calcium-independent cell adhesion molecule influencing cell cycle, proliferation, and metabolism through induction of c-myc, cyclin A, and cyclin E genes.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth.
Overexpression is associated with enhanced epithelial proliferation and is a marker for tumors of epithelial origin.
Ep-CAM is expressed on the basolateral surface of most simple epithelia and many carcinoma types, and helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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