Thermo Fisher Scientific EPCAM Monoclonal Antibody (OTI1H2), TrueMAB
EpCAM 단백질을 인식하는 마우스 단클론 항체로, Western blot 및 IHC(Paraffin) 분석에 적합합니다. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다. EpCAM 관련 암 연구 및 세포 접착 분자 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H2 |
| Immunogen | Full length human recombinant protein of human EPCAM produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues.
It functions as a calcium-independent cell adhesion molecule influencing cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and regulatory genes cyclin A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth.
Overexpression of Ep-CAM is linked to increased epithelial proliferation and is a marker for epithelial-origin tumors.
It is expressed on the basolateral surface of most simple epithelia and many carcinoma types and helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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