Thermo Fisher Scientific Pierce Recombinant Protein A/G, Alkaline Phosphatase Conjugated

Thermo Fisher Scientific Pierce Recombinant Protein A/G, Alkaline Phosphatase Conjugated

Use Thermo Scientific Pierce Recombinant Alkaline Phosphatase Conjugated Protein A/G to probe and detect antibodies, especially IgG isotypes, from various species in Western blotting, ELISA, IHC, and other immunoassay protocols.

Features of Recombinant Protein A/G

• Contains four Fc-binding domains from Protein A and two from Protein G
• Provides binding for all antibody species and subclasses recognized by either Protein A or Protein G

The extended Fc-binding properties of Protein A/G make it a popular tool for investigating and purifying immunoglobulins. Protein A/G binds to all human IgG subclasses, IgA, IgE, IgM, and to a lesser extent IgD; however, it does not bind mouse IgA, IgM, or murine serum albumin.
This chimeric protein is ideal for purification and detection of mouse monoclonal antibodies from IgG subclasses without interference from other serum proteins. Individual subclasses of mouse monoclonals often show stronger affinity to Protein A/G than to either Protein A or Protein G alone.

Properties of Recombinant Protein A/G

Gene fusion of the Fc-binding domains of Protein A and Protein G produces a structurally and functionally chimeric protein with broader binding capacity than either Protein A or Protein G alone.
During fusion, the Protein G gene sequence coding for the serum albumin-binding site is removed. The protein is expressed in E. coli and secreted into the surrounding medium during fermentation.
The bacterial host is engineered to be deficient in major proteolytic activities, ensuring consistent product quality and yield.
Binding occurs well at pH 5–8 and is less pH-dependent than either Protein A or Protein G alone.

Related Products

• Pierce™ Recombinant Protein A/G
• Pierce™ Recombinant Protein A/G, Peroxidase Conjugated

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