Thermo Fisher Scientific F(ab`)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, APC

Applications

Tested Dilution

Immunohistochemistry (IHC)

1:50 - 1:200

Immunocytochemistry (ICC/IF)

0.5 mg/mL

Flow Cytometry (Flow)

1:500

Immunoprecipitation (IP)

1:50 - 1:200

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

APC APC APC

View additional formats

• [Alexa Fluor 350 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 350/host/Goat/)
• [Alexa Fluor 405 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 405/host/Goat/)
• [Alexa Fluor 430 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 430/host/Goat/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Goat/)
• [Alexa Fluor 514 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 514/host/Goat/)
• [Alexa Fluor 532 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 532/host/Goat/)
• [Alexa Fluor 546 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 546/host/Goat/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 555/host/Goat/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 568/host/Goat/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Goat/)
• [Alexa Fluor 633 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 633/host/Goat/)
• [Alexa Fluor 635 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 635/host/Goat/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Goat/)
• [Alexa Fluor 660 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 660/host/Goat/)
• [Alexa Fluor 680 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 680/host/Goat/)
• [Alexa Fluor 700 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 700/host/Goat/)
• [Alexa Fluor 750 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 750/host/Goat/)
• [Alexa Fluor 790 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 790/host/Goat/)
• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor Plus 488/host/Goat/)
• [Alexa Fluor Plus 555 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor Plus 555/host/Goat/)
• [Alexa Fluor Plus 594 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor Plus 594/host/Goat/)
• [Alexa Fluor Plus 647 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor Plus 647/host/Goat/)
• [AP](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/AP/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Biotin/host/Goat/)
• [Biotin-XX](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Biotin-XX/host/Goat/)
• [Cyanine3](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Cyanine3/host/Goat/)
• [Cyanine5 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Cyanine5/host/Goat/)
• [DyLight 350](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 350/host/Goat/)
• [DyLight 405](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 405/host/Goat/)
• [DyLight 488](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 488/host/Goat/)
• [DyLight 550](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 550/host/Goat/)
• [DyLight 594](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 594/host/Goat/)
• [DyLight 633](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 633/host/Goat/)
• [DyLight 650](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 650/host/Goat/)
• [DyLight 680](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 680/host/Goat/)
• [DyLight 755](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 755/host/Goat/)
• [DyLight 800](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 800/host/Goat/)
• [FITC](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/HRP/host/Goat/)
• [Pacific Blue](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Pacific Blue/host/Goat/)
• [Pacific Orange](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Pacific Orange/host/Goat/)
• [PE ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/PE/host/Goat/)
• [PE-Alexa Fluor 610](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/PE-Alexa Fluor 610/host/Goat/)
• [PE-Alexa Fluor 647](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/PE-Alexa Fluor 647/host/Goat/)
• [Rhodamine](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Rhodamine/host/Goat/)
• [Rhodamine Red-X](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Rhodamine Red-X/host/Goat/)
• [Texas Red](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Texas Red/host/Goat/)
• [Texas Red-X](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Texas Red-X/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/TRITC/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

651/660 nm View spectra

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 15mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_429727

Target

IgG

Cross Adsorption

Against human, mouse and rat serum proteins

Antibody Form

F(ab`)2 Fragment

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

Product # 31984 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.

Product # 31984 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, mouse, and rat serum proteins. However, this antibody may cross-react with immunoglobulins from other species.

Phycobiliprotein Concentration: 0.5 mg/mL (determined by absorption = 73 at 650 nm for a

1% solution for only those APC molecules to which at least one molecule of active antibody is bound)

Allophycocyanin Source: Spirulina platensis

Store product protected from light at 4°C until opened. Product may be stored for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day. Do not freeze. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Allophycocyanin Amax= 650 nm; Emax= 660 nm.

Reconstitute with 0.5 mL of distilled water (0.5 mg/mL after restoration).

Country of Origin: USA

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.