Thermo Fisher Scientific SIRPA Monoclonal Antibody (1D10)

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

ELISA

• Tested Dilution: 10 µg/mL

Product Specifications

Product Specific Information

Sequence of this protein is as follows:

VAGEEELQVI QPDKSVSVAA GESAILHCTV TSLIPVGPIQ WFRGAGPARE LIYNQKEGHF PRVTTVSEST KRENMDFSIS ISNITPADAG TYYCVKFRKG SPDTEFKSGA GTELSVRAKP SAPVVSGPAA RATPQHTVSF TCESHGFSPR DITLKWFKNG NELSDFQTNV DPVGESVSYS IHSTAKVVLT REDVHSQVIC EVAHVTLQGD PLRGTANLSE TIRVPPTLEV TQQPVRAENQ VNVTCQVRKF YPQRLQLTWL ENGNVSRTET ASTVTENKDG TYNWMSWLLV NVSAHRDDVK LTCQVEHDGQ PAVSKSHDLK VSAHPKEQGS NTAAENTGSN ERNIYIVVGV VCTLLVALLM AALYLVRIRQ KKAQGSTSST RLHEPEKNAR EITQVQSLDT NDITYADLNL PKGKKPAPQA AEPNNHTEYA SIQTSPQPAS EDTLTYADLD MVHLNRTPKQ PAPKPEPSFS EYASVQVPRK

Target Information

SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and hematopoietic stem cells.
SIRP alpha acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways.

The ligand binding of SIRP alpha to integrin-associated protein CD47 results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of SIRP alpha, which mediates the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2.

Ligation of SIRP alpha with CD47 has been demonstrated in several regulatory processes, including:

• Inhibition of host cell phagocytosis by macrophages
• Bi-directional activation of T cells and DCs

SIRP alpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones, and cell adhesion, and plays a general role in different physiological and pathological processes.
Cancer cells highly express CD47, which activates SIRP alpha and inhibits macrophage-mediated destruction of cancerous cell growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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