Thermo Fisher Scientific SIRPA Monoclonal Antibody (4C7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

ELISA (ELISA)

0.1 ng/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

4C7

Immunogen

SIRPA (AAH26692, 28 a.a. approximately 507 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SIRP alpha (CD172a),uniProtId:P78324-1,ncbiNodeId:9606,antigenRange:1-504,antigenLength:504,antigenImageFileName:H00140885-M10_SIRP_alpha_CD172a_P78324-1_House_mouse.svg,antigenImageFileNamePDP:H00140885-M10_SIRP_alpha_CD172a_P78324-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: VAGEEELQVI QPDKSVSVAA GESAILHCTV TSLIPVGPIQ WFRGAGPARE LIYNQKEGHF PRVTTVSEST KRENMDFSIS ISNITPADAG TYYCVKFRKG SPDTEFKSGA GTELSVRAKP SAPVVSGPAA RATPQHTVSF TCESHGFSPR DITLKWFKNG NELSDFQTNV DPVGESVSYS IHSTAKVVLT REDVHSQVIC EVAHVTLQGD PLRGTANLSE TIRVPPTLEV TQQPVRAENQ VNVTCQVRKF YPQRLQLTWL ENGNVSRTET ASTVTENKDG TYNWMSWLLV NVSAHRDDVK LTCQVEHDGQ PAVSKSHDLK VSAHPKEQGS NTAAENTGSN ERNIYIVVGV VCTLLVALLM AALYLVRIRQ KKAQGSTSST RLHEPEKNAR EITQVQSLDT NDITYADLNL PKGKKPAPQA AEPNNHTEYA SIQTSPQPAS EDTLTYADLD MVHLNRTPKQ PAPKPEPSFS EYASVQVPRK

Target Information

SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and hematopoietic stem cells. SIRP alpha acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. The ligand binding of SIRP alpha to integrin-associated protein CD47 results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of SIRP alpha, which mediates the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. Ligation of SIRP alpha with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs. SIRP alpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion, and plays a general role in different physiological and pathological processes. Cancer cells highly express CD47, which activates SIRP alpha and inhibits macrophage-mediated destruction of cancerous cell growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.