Thermo Fisher Scientific VEGF Polyclonal Antibody
Rabbit polyclonal antibody recognizing human VEGF, suitable for IHC(P) and ICC/IF applications. Recommended for angiogenesis and tumor research. Antigen affinity purified, liquid form, stored at 4°C or -20°C. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from N-terminus of human VEGF |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1088220 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 1 mM EDTA buffer (pH 8.0) for 10 minutes, followed by cooling at room temperature for 20 minutes. After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature. A suggested positive control is angiosarcoma or placenta tissue.
Target Information
VEGF (vascular endothelial growth factor) is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis that promotes tumor progression and metastasis. VEGF influences vascular endothelium by promoting endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability.
The VEGF family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system (FLT1/VEGFR-1 and FLK1/KDR/VEGFR-2) are fundamental regulators of angiogenesis and are expressed mainly on endothelial cells.
Five isoforms of VEGF are generated by alternative splicing from a single VEGF gene. An alternately spliced form of FLT1 produces a soluble protein (sFLT1) that binds VEGF with high affinity, inhibiting angiogenesis.
Elevated VEGF levels are linked to POEMS (Crow-Fukase) syndrome, which affects multiple organs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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