Thermo Fisher Scientific alpha Tubulin Polyclonal Antibody
Rabbit polyclonal antibody against alpha Tubulin for WB, IHC, and ICC applications. Reacts with human, mouse, rat, and dog. Affinity-purified, liquid form, stored at -20°C. Suitable for microtubule structure and cytoskeletal studies.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:4,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Dog |
| Published Species | Mouse, Sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from C-terminus of human tubulin alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2210051 |
Product Specific Information
This antibody is predicted to react with mouse, bovine, guinea pig, porcine, gerbil, and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended before staining using 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is lung tissue.
Target Information
Alpha Tubulin forms a heterodimer with beta Tubulin, each approximately 55 kDa. This heterodimer is the fundamental unit of microtubules, cylindrical filamentous structures found in nearly all eukaryotic cells.
Microtubules provide structural support, serve as intracellular transport tracks, and play essential roles in mitosis.
Alpha and beta tubulins are highly conserved across species, with gamma Tubulin involved in microtubule nucleation.
Alpha Tubulin gene expression is mainly observed in morphologically differentiated neurological cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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