Takara Luciferase reporters—Ready-To-Glow secreted luciferase assay

Overview

• Secreted luciferase assay protocol eliminates cell lysis
• 2–4 fold higher signal than firefly or Renilla luciferase
• Single transfection allows multiple time points from the same cell
• Dual, live-cell assay includes SEAP as well as secreted luciferase:
  monitor two promoters, or use one reporter as a normalization control

Applications

• Homogeneous assay protocols
• Time-course studies
• Test multiple compounds
• Suitable for downstream experiments with the same cells
• Use the dual assay (which includes secreted alkaline phosphatase as well as a secreted luciferase assay) to monitor two promoters
• Use the dual assay to monitor one promoter with a built-in normalization control

Additional Product Information

Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications.
Refer to the Kit Components List to determine kit components.
Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Specifications