Thermo Fisher Scientific C14orf126 Polyclonal Antibody
C14orf126 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. IHC(P) 및 ICC/IF에 적합하며 인간 시료에 반응. 항원 친화 크로마토그래피로 정제되었으며 PBS 기반 완충액에 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:50 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human C14orf126 (Product #RP-88856) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2638752 |
Product Specific Information
Immunogen sequence:
WVEVQRGLVI YVCFFKGADK ELLPKMVNTL LNVKLSETEN GKHVSILDLP GNILIIPQAT LGGRLKGRNM QYHSNSGKEE GFELYSQFVT LCEKEVAANS KCAEARVVVE HGTYGNRQVL KLDTNGPFTH LIFE
Highest antigen sequence identity to the following orthologs:
- Mouse: 84%
- Rat: 85%
Target Information
Deacylates mischarged D-aminoacyl-tRNAs and mischarged glycyl-tRNA(Ala), protecting cells from glycine mischarging by AlaRS. Functions by rejecting L-amino acids from its binding site rather than specifically recognizing D-amino acids. Catalyzes hydrolysis of D-tyrosyl-tRNA(Tyr) but not correctly charged L-tyrosyl-tRNA(Tyr).
By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, it prevents toxicity associated with D-aminoacyl-tRNA formation and maintains protein L-homochirality.
Unlike DTD1, it can deacylate L-Ala mischarged on tRNA(Thr)(G4.U69) due to relaxed substrate chirality specificity caused by the trans conformation of the Gly-Pro motif in the active site (PubMed:29410408).
Also hydrolyzes correctly charged glycyl-tRNA(Gly) in vitro, though in vivo EEF1A1/EF-Tu may protect it from DTD2-mediated deacetylation. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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