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Thermo Fisher Scientific Goat anti-Human IgG (H+L) Secondary Antibody, Rhodamine
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Thermo Fisher Scientific Goat anti-Human IgG (H+L) Secondary Antibody, Rhodamine

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인간 IgG(H+L)를 인식하는 염소 유래 로다민 결합 2차 항체로, IHC, ICC/IF, Flow, IP 등 다양한 면역실험에 적합합니다. 높은 특이성과 형광 감도를 제공하며, 4°C 보관 및 빛 차단이 필요합니다.

카탈로그번호
31656
판매단위
pk
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마지막 업데이트 2025. 08. 01. 오후 07:45
Thermo Fisher Scientific 31656 Goat anti-Human IgG (H+L) Secondary Antibody, Rhodamine 2 mg pk판매 단위 pk ·
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256,200원VAT 포함 281,820원

Thermo Fisher Scientific · Thermo Fisher Scientific Goat anti-Human IgG (H+L) Secondary Antibody, Rhodamine

Thermo Fisher Scientific Goat anti-Human IgG (H+L) Secondary Antibody, Rhodamine

Applications and Tested Dilution

Application Tested Dilution
Immunohistochemistry (IHC) 1:50 - 1:200
Immunocytochemistry (ICC/IF) 1:50 - 1:200
Flow Cytometry (Flow) 1:50 - 1:200
Immunoprecipitation (IP) 1:50 - 1:200

Product Specifications

Specification Description
Species Reactivity Human
Host/Isotype Goat / IgG
Class Polyclonal
Type Secondary Antibody
Conjugate Rhodamine
Excitation/Emission Max 573/591 nm
Form Lyophilized
Concentration 1.5 mg/mL
Purification Affinity chromatography
Storage Buffer PBS, pH 7.6, with 15 mg/mL BSA
Contains 0.05% sodium azide
Storage Conditions 4°C, protected from light
Shipping Conditions Ambient (domestic); Wet ice (international)
RRID AB_228270
Target IgG
Antibody Form Whole Antibody
Country of Origin USA
  • Alexa Fluor 488
  • Alexa Fluor 647
  • Alexa Fluor Plus 488
  • Alexa Fluor Plus 647
  • AP
  • Biotin
  • FITC
  • HRP
  • PE
  • Texas Red
  • TRITC
  • Custom conjugation available

Product Specific Information

  • Concentration may vary slightly by lot; refer to datasheet for exact concentration.
  • Product #31656 validated for Western blot, IF, ICC, IHC, IP, and FACS applications.
  • Reacts with heavy chains of human IgG and light chains common to most human immunoglobulins; minimal cross-reactivity with non-immunoglobulin serum proteins.
  • May cross-react with immunoglobulins from other species.
  • Store protected from light at 4°C.
  • To extend shelf-life, add equal volume of glycerol (final 50%) and store at -20°C.
  • Rhodamine Amax = 550 nm; Emax = 570 nm; Fluorophore/Protein Absorbance Ratio A550/A280 ≈ 0.4 (lot-dependent).
  • Reconstitute with 1.5 mL distilled water (final 1.5 mg/mL).

Target Information

Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins.
They are used for detection, sorting, or purification of human targets.
Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically generated by immunizing host animals with pooled immunoglobulins from the target species and purified via immunoaffinity chromatography or conjugation modifications.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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