Thermo Fisher Scientific DNMT3A Monoclonal Antibody (64B1446)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• In Western blot, detects a band at approximately 120 kDa (predicted molecular weight: 102 kDa).
• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer (pH 6.0) for 10–20 min, followed by cooling at room temperature for 20 min.
• Suggested positive control: antigen standard for DNMT3A (transient overexpression lysate), transfected cell lysate (WB), HeLa cells (ICC).

Target Information

Methylation of DNA at cytosine residues plays an important role in the regulation of gene expression, genomic imprinting, and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with cancer development.

Three families of mammalian DNA methyltransferase genes have been identified: Dnmt1, Dnmt2, and Dnmt3.

• Dnmt1: Constitutively expressed in proliferating cells; its inactivation causes global demethylation and embryonic lethality.
• Dnmt2: Expressed at low levels in adult tissues; its inactivation does not affect DNA methylation.
• Dnmt3 family (Dnmt3a, Dnmt3b): Strongly expressed in embryonic stem (ES) cells, downregulated during differentiation, and low in adult somatic tissues.
• Mutations in Dnmt3b are associated with ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.